Glycerol Kinase antibody (Biotin)

Catalog No. ABIN459044

The Rabbit Polyclonal anti-Glycerol Kinase antibody has been validated for ELISA, WB and IF. It is suitable to detect Glycerol Kinase in samples from Bacillus stearothermophilus.

Quick Overview for Glycerol Kinase antibody (Biotin) (ABIN459044)

Target: Glycerol Kinase (GK)

Reactivity: Bacillus stearothermophilus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glycerol Kinase antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Glycerol Kinase Antibody (Biotin)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to glycerokinase from Bacillus stearothermophilus

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Glycerkinase isolated and purified from Bacillus stearothermophilus. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 4.6. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is rec onstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Glycerol Kinase

Target: Glycerol Kinase (GK)

Alternative Name: Glycerokinase

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.