PLA2G1B antibody (Biotin)

Catalog No. ABIN459092

The Rabbit Polyclonal anti-PLA2G1B antibody has been validated for WB, ELISA and IF. It is suitable to detect PLA2G1B in samples from Rattlesnake.

Quick Overview for PLA2G1B antibody (Biotin) (ABIN459092)

Target: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Reactivity: Rattlesnake

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLA2G1B antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-PLA2G1B Antibody (Biotin)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phospholipase A 2 from Crotalus durissus terrificus venom

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Phospholipase A 2 isolated and purified from Crotalus durissus terrificus venom. Freund’s adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 4.7. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C . It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for PLA2G1B

Target: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Alternative Name: Phospholipase A 2

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Pathways: Inositol Metabolic Process, VEGF Signaling