Ribonuclease T1 antibody (Biotin)

Catalog No. ABIN459107

The Rabbit Polyclonal anti-Ribonuclease T1 antibody has been validated for IF, ELISA and WB. It is suitable to detect Ribonuclease T1 in samples from Aspergillus oryzae.

Quick Overview for Ribonuclease T1 antibody (Biotin) (ABIN459107)

Target: Ribonuclease T1

Reactivity: Aspergillus oryzae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ribonuclease T1 antibody is conjugated to Biotin

Application: Immunofluorescence (IF), ELISA, Western Blotting (WB)

Product Details anti-Ribonuclease T1 Antibody (Biotin)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to ribonuclease T1 from Aspergillus oryzae

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Ribonuclease T1 isolated and purified from Aspergillus oryzae. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.1. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prio r to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the s ame day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Ribonuclease T1

Target: Ribonuclease T1

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.