Nuclease antibody

Catalog No. ABIN459242

The Rabbit Polyclonal anti-Nuclease antibody has been validated for ELISA, WB and IF. It is suitable to detect Nuclease in samples from Staphylococcus aureus.

Quick Overview for Nuclease antibody (ABIN459242)

Target: Nuclease

Reactivity: Staphylococcus aureus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nuclease antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Nuclease Antibody

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: IgG fraction of polyclonal rabbit antiserum to nuclease from Staphylococcus aureus

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Nuclease isolated and purified from Staphylococcus aureus. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice. Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/10,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Nuclease

Target: Nuclease

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.