Nuclease antibody (Biotin)

Catalog No. ABIN459080

Product Details anti-Nuclease Antibody (Biotin)

Target: Nuclease

Reactivity: Staphylococcus aureus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nuclease antibody is conjugated to Biotin

Application: Immunofluorescence (IF), ELISA, Western Blotting (WB)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to nuclease from Staphylococcus aureus

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Nuclease isolated and purified from Staphylococcus aureus. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 4.8. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Nuclease

Target: Nuclease

Abstract: Nuclease Products

Synonyms: F15D2.37 antibody, F15D2_37 antibody, 5'-3' exonuclease family protein antibody, Nuclease antibody, AT1G29630 antibody, Nuc antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.