Caspase 3 antibody

Catalog No. ABIN462092

The Rabbit Polyclonal anti-Caspase 3 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Caspase 3 in samples from Human, Mouse, Rat, Cow, Dog, Rabbit, Monkey, Pig, Guinea Pig, Hamster, Chicken and Saccharomyces cerevisiae. There are 2+ publications available.

Quick Overview for Caspase 3 antibody (ABIN462092)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Monkey, Pig, Guinea Pig, Hamster, Chicken, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 3 Antibody

Brand: IHC-plus™

Purification: Protein A purified

Immunogen: Recombinant human Caspase-3 protein (full length).

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

References for anti-Caspase 3 antibody (ABIN462092)

Baumbach, Schrödl, Nenoff, Uhrlaß, Mülling, Michler: "Modeling dermatophytosis: Guinea pig skin explants represent a highly suitable model to study Trichophyton benhamiae infections." in: The Journal of dermatology, Vol. 47, Issue 1, pp. 8-16, (2020) (PubMed).

Domian, Surażyński, Szarmach, Piotrowska, Kasacka: "Mechanism of pro-apoptotic action of prosthetic restorations on oral mucosa cells." in: Advances in medical sciences, Vol. 65, Issue 1, pp. 134-140, (2020) (PubMed).

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3 / Caspase 3

Background: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation