CRABP1 antibody (AA 1-137)

Catalog No. ABIN462276

This anti-CRABP1 antibody is a Mouse Monoclonal antibody detecting CRABP1 in WB, ELISA, IHC and IHC (p). Suitable for Human and Mouse.

Quick Overview for CRABP1 antibody (AA 1-137) (ABIN462276)

Target: CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRABP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AT1A1

Product Details anti-CRABP1 Antibody

Binding Specificity: AA 1-137

Specificity: Human CRABP1 (1-137aa)

Purification: Protein G purified

Immunogen: Recombinant human CRABP1 (1-137aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Extracts of mouse eye (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for CRABP1

Target: CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Alternative Name: CRABP1 / CRABP

Background: Name/Gene ID: CRABP1

Synonyms: CRABP1, CRABP-I, CRABPI, CRABP

Gene ID: 1381

UniProt: P29762