CLIP1 antibody (AA 1404-1417)

Catalog No. ABIN462301

This Goat Polyclonal antibody specifically detects CLIP1 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CLIP1 antibody (AA 1404-1417) (ABIN462301)

Target: CLIP1 (CAP-GLY Domain Containing Linker Protein 1 (CLIP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CLIP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CLIP1 Antibody

Binding Specificity: AA 1404-1417

Specificity: Human CLIP1 / CLIP170. This antibody is expected to recognize both reported isoforms (NP_002947.1, NP_937883.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum (93%) Turkey, Chicken, Pufferfish (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-PHSTHHGSRGEERP from the C-terminus of human CLIP1 / CLIP170 (NP_002947.1, NP_937883.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum (93%), Turkey, Chicken, Pufferfish (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:8000, 1 - 3 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CLIP1

Target: CLIP1 (CAP-GLY Domain Containing Linker Protein 1 (CLIP1))

Alternative Name: CLIP1 / CLIP-170

Background: Name/Gene ID: CLIP1

Synonyms: CLIP1, CLIP, CLIP-170, CYLN1, Cytoplasmic linker protein 1, Restin, CLIP170, RSN

Gene ID: 6249

NCBI Accession: NP_002947, NP_937883

UniProt: P30622

Pathways: Microtubule Dynamics