ALPL antibody

Catalog No. ABIN462352

This Mouse Monoclonal antibody specifically detects ALPL in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human and Cow.

Quick Overview for ALPL antibody (ABIN462352)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ALPL Antibody

Brand: IHC-plus™

Specificity: Recognizes Alkaline Phosphatase from human bone. Species cross-reactivity: Alkaline phosphatase from human placenta and calf intestine.

Purification: Protein A purified

Immunogen: Purified alkaline phosphatase (native) from human bone.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Suitable for use in ELISA and Western Blot. Western Blot: Stains a band of bone AP at ~90kD.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: ALPL / Alkaline Phosphatase

Background: Name/Gene ID: ALPL

Synonyms: ALPL, Alkaline phosphomonoesterase, AP-TNAP, Alkaline phosphatase liver, APTNAP, Glycerophosphatase, Tissue-nonspecific ALP, TNAP, HOPS, Liver alkaline phosphatase, TNSALP

Gene ID: 249

UniProt: P05186