Caspase 7 antibody

Catalog No. ABIN462356

The Mouse Monoclonal anti-Caspase 7 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Caspase 7 in samples from Human, Mouse, Rat, Monkey, Pig, Dog, Rabbit, Hamster and Sheep.

Quick Overview for Caspase 7 antibody (ABIN462356)

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Reactivity: Human, Mouse, Rat, Monkey, Pig, Dog, Rabbit, Hamster, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7-1-11

Product Details anti-Caspase 7 Antibody

Brand: IHC-plus™

Specificity: Detects an ~37 kD protein, corresponding to the apparent molecular mass of the caspase-7 on SDS-PAGE immunoblots, in samples from human, mouse, rat, monkey, dog, sheep, rabbit, pig and hamster origins. This antibody recognizes the unprocessed pro-caspase-7 (p35), caspase-7 lacking the short amino- terminal pro domain (32 kD) and the fully processed caspase-7 (p19) in apoptotic cell extracts.

Purification: Protein G purified

Immunogen: Recombinant human caspase-7 full length protein.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (10 mM sodium phosphate,  pH 7.2, 50 mM sodium chloride, 0.1 mM PMSF). Sourced in Ascites.

Preservative: PMSF

Handling Advice: avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: CASP7 / Caspase 7

Background: Name/Gene ID: CASP7
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP7, Apoptotic protease Mch-3, Caspase 7, Caspase-7, ICE-LAP3, LICE2, MCH3, ICE-like apoptotic protease 3, CASP-7, CMH-1

Gene ID: 840

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity