AKT1/2 antibody

Catalog No. ABIN462374

The Rabbit Polyclonal anti-AKT1/2 antibody has been validated for WB, IHC, IP and IHC (p). It is suitable to detect AKT1/2 in samples from Human, Mouse and Rat.

Quick Overview for AKT1/2 antibody (ABIN462374)

Target: AKT1/2 (V-Akt Murine Thymoma Viral Oncogene Homolog 1/2 (AKT1/2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AKT1/2 Antibody

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the sequence of human Akt, conjugated to KLH, sequence identical with rat and bovine.

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), IP, WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for AKT1/2

Target: AKT1/2 (V-Akt Murine Thymoma Viral Oncogene Homolog 1/2 (AKT1/2))

Alternative Name: AKT1 + AKT2

Background: Name/Gene ID: AKT1/AKT2

Synonyms: AKT1/AKT2

This antibody detects: AKT1 + AKT2