CRYAB antibody

Catalog No. ABIN462382

This Rabbit Polyclonal antibody specifically detects CRYAB in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Cow and Pig.

Quick Overview for CRYAB antibody (ABIN462382)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CRYAB Antibody

Purification: Protein A purified

Immunogen: Synthetic peptide derived from sequence near the carboxy-terminus of human alphaB-Crystallin, conjugated to KLH, sequence identical to rat, rabbit, and bovine.

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: CRYAB / Alpha B Crystallin

Background: Name/Gene ID: CRYAB

Synonyms: CRYAB, Alpha-crystallin B chain, CRYA2, Crystallin, alpha B, CTPP2, Heat shock protein beta-5, HSPB5, Alpha B Crystallin, Alpha(B)-crystallin, Rosenthal fiber component, Heat-shock 20 kD like-protein

Gene ID: 1410

UniProt: P02511