Integrin beta 4 antibody

Catalog No. ABIN462464

The Rat Monoclonal anti-Integrin beta 4 antibody is suitable to detect Integrin beta 4 in samples from Human. It has been validated for WB, IHC, FACS, IHC (fro) and IHC (p).

Quick Overview for Integrin beta 4 antibody (ABIN462464)

Target: Integrin beta 4 (ITGB4)

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 439-9B

Product Details anti-Integrin beta 4 Antibody

Purification: Affinity purified

Immunogen: Human ITGB4

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo, IHC, IHC-Fr, IHC-P (20 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL. Tested by flow cytometric analysis of A549 cell line. Antibody can be used at less than or equal to 1 μg per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer.. Sourced from tissue culture supernatant

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing and store undiluted.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: ITGB4 / Integrin Beta 4

Background: Name/Gene ID: ITGB4
Family: Integrin

Synonyms: ITGB4, CD104, Integrin beta-4, Integrin Beta 4, gp150, CD104 antigen, Integrin beta-4 subunit, Integrin, beta 4

Gene ID: 3691

UniProt: P16144

Pathways: Integrin Complex