CD163 antibody

Catalog No. ABIN462466

This Mouse Monoclonal antibody specifically detects CD163 in WB, IHC, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD163 antibody (ABIN462466)

Target: CD163

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GHI-61

Product Details anti-CD163 Antibody

Specificity: CD163

Purification: Affinity purified

Immunogen: Spleen cells from a case of hairy cell leukemia

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (20 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL. Tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, <=0.09 % sodium azide. Sourced from tissue culture supernatant.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing and store undiluted.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background: Name/Gene ID: CD163

Synonyms: CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 Molecule, Hemoglobin scavenger receptor, MM130

Gene ID: 9332

UniProt: Q86VB7