DC-SIGN/CD209 antibody

Catalog No. ABIN462467

The Rat Monoclonal anti-DC-SIGN/CD209 antibody has been validated for FACS, IHC, IP and IHC (p). It is suitable to detect DC-SIGN/CD209 in samples from Human.

Quick Overview for DC-SIGN/CD209 antibody (ABIN462467)

Target: DC-SIGN/CD209 (CD209) (CD209)

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This DC-SIGN/CD209 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: H209

Product Details anti-DC-SIGN/CD209 Antibody

Brand: IHC-plus™

Purification: Affinity purified

Immunogen: Human CD209

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (20 μg/mL), IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL. Tested by flow cytometric analysis of cultured human dendritic cells and peripheral blood leukocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing and store undiluted.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for DC-SIGN/CD209

Target: DC-SIGN/CD209 (CD209) (CD209)

Alternative Name: CD209 / DC-SIGN

Background: Name/Gene ID: CD209

Synonyms: CD209, CD209 antigen, CD209 Molecule, CDSIGN, CLEC4L, DC-SIGN, HIV gpl20-binding protein, DC-SIGN1

Gene ID: 30835

UniProt: Q9NNX6