MFN1 antibody (AA 745-756)

Catalog No. ABIN462500

The Goat Polyclonal anti-MFN1 antibody has been validated for IHC and IHC (p). It is suitable to detect MFN1 in samples from Human, Rabbit, Dog, Horse and Monkey.

Quick Overview for MFN1 antibody (AA 745-756) (ABIN462500)

Target: MFN1 (Mitofusin 1 (MFN1))

Reactivity: Human, Rabbit, Dog, Horse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This MFN1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MFN1 Antibody

Binding Specificity: AA 745-756

Brand: IHC-plus™

Specificity: Human MFN1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Elephant, Dog, Horse, Rabbit (100%) Gorilla, Mouse, Rat, Bovine (92%) Bat, Platypus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NKAVQLENELEN from an internal region of human MFN1 (NP_284941.2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Elephant, Dog, Horse, Rabbit (100%), Gorilla, Mouse, Rat, Bovine (92%), Bat, Platypus (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MFN1

Target: MFN1 (Mitofusin 1 (MFN1))

Alternative Name: MFN1

Background: Name/Gene ID: MFN1

Synonyms: MFN1, Fzo homolog, Hfzo2, Mitofusin-1, Hfzo1, Mitofusin 1, Putative transmembrane GTPase, Transmembrane GTPase MFN1

Gene ID: 55669

NCBI Accession: NP_284941

UniProt: Q8IWA4