RASSF2 antibody (AA 11-22)

Catalog No. ABIN462512

The Goat Polyclonal anti-RASSF2 antibody has been validated for IHC (p) and IHC. It is suitable to detect RASSF2 in samples from Human, Monkey, Cow, Dog, Horse, Rabbit and Pig.

Quick Overview for RASSF2 antibody (AA 11-22) (ABIN462512)

Target: RASSF2 (Ras Association (RalGDS/AF-6) Domain Family Member 2 (RASSF2))

Reactivity: Human, Monkey, Cow, Dog, Horse, Rabbit, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This RASSF2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Product Details anti-RASSF2 Antibody

Binding Specificity: AA 11-22

Brand: IHC-plus™

Specificity: Human RASSF2. Reported variants represent identical protein: NP_055552.1, NP_739580.1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%) Bat, Xenopus (92%) Chicken, Frog (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide PCGQDKYISKNE from the N-terminus of human RASSF2 (NP_055552.1, NP_739580.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%), Bat, Xenopus (92%), Chicken, Frog (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RASSF2

Target: RASSF2 (Ras Association (RalGDS/AF-6) Domain Family Member 2 (RASSF2))

Alternative Name: RASSF2

Background: Name/Gene ID: RASSF2

Synonyms: RASSF2, CENP-34, KIAA0168, RASFADIN, Centromere protein 34

Gene ID: 9770

NCBI Accession: NP_055552, NP_739580

UniProt: P50749