CCL22 antibody (Biotin)

Catalog No. ABIN464840

This Rabbit Polyclonal antibody specifically detects CCL22 in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for CCL22 antibody (Biotin) (ABIN464840)

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL22 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL22 Antibody (Biotin)

Specificity: Mouse MDC

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine MDC.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mMDC by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mMDC. To detect mMDC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine MDC (LS-C104280) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMDC. Western Blot: To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL22

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Alternative Name: MDC / CCL22

Background: Name/Gene ID: CCL22
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL22, A-152E5.1, ABCD-1, CC chemokine STCP-1, DC/B-CK, Macrophage-derived chemokine, MDC(1-69), MDC, SCYA22, Small inducible cytokine A22, STCP-1, C-C motif chemokine 22, Small-inducible cytokine A22

Gene ID: 6367

UniProt: O00626