Interleukin 17a antibody (Biotin)

Catalog No. ABIN464998

This anti-Interleukin 17a antibody is a Goat Polyclonal antibody detecting Interleukin 17a in ELISA, WB and Neut. Suitable for Human.

Quick Overview for Interleukin 17a antibody (Biotin) (ABIN464998)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Interleukin 17a antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Product Details anti-Interleukin 17a Antibody (Biotin)

Specificity: Human IL-17A

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-17A.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hIL-17A by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-17A (LS-C104427) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid repeated freeze-thaw

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least 2 weeks at 2-8°C. Frozen aliquots are stable for at least 6 months at -20°C. Avoid freeze-thaw cycles.

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL17A / IL17

Background: Name/Gene ID: IL17A
Family: Interleukin

Synonyms: IL17A, CTLA-8, CTLA8, Interleukin-17A, Interleukin 17A, IL-17, IL-17A, IL17

Gene ID: 3605

UniProt: Q16552