CCL18 antibody (Biotin)

Catalog No. ABIN465014

This Rabbit Polyclonal antibody specifically detects CCL18 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for CCL18 antibody (Biotin) (ABIN465014)

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL18 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL18 Antibody (Biotin)

Specificity: Human MIP-4

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIP-4.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, SwELISA, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hMIP-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-4. To detect hMIP-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-4 (LS-C104536) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-4. Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage, up to 1 year. Avoid freeze/thaw cycles.

Target Details for CCL18

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Alternative Name: CCL18 / PARC

Background: Name/Gene ID: CCL18
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL18, AMAC1, Cc chemokine amac1, DC-CK1, Chemokine (C-C), dendritic, DCCK1, MIP-4, AMAC-1, MIP4, SCYA18, Small-inducible cytokine A18, C-C motif chemokine 18, Cc chemokine (amac)-1, CC chemokine ligand 18, CC chemokine PARC, CKb7, Dendritic cell chemokine 1, PARC, Small inducible cytokine A18

Gene ID: 6362

UniProt: P55774