BMP4 antibody

Catalog No. ABIN465142

This Mouse Monoclonal antibody specifically detects BMP4 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for BMP4 antibody (ABIN465142)

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-BMP4 Antibody

Specificity: Human BMP-4

Purification: Protein G purified

Immunogen: E.coli derived recombinant mature Human BMP-4.

Type of Immunogen: Cell extract

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (4 - 8 μg/mL), Neut (0.4 - 0.6 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained HepG2 cells. The primary antibody was incubated at 3 μg/mL overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP-4 (5 ng/mL), a concentration of 0.4-0.6 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human BMP-4 when used in conjunction with compatible secondary reagents. Western Blot: To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2-4 ng/lane, under non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for BMP4

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Alternative Name: BMP4 / BMP-4

Background: Name/Gene ID: BMP4
Family: TGF beta

Synonyms: BMP4, BMP-2B, BMP-4, BMP2B, BMP2B1, Bone morphogenetic protein 2B, Bone morphogenetic protein 4, DVR4, MCOPS6, OFC11, Zyme

Gene ID: 652

UniProt: P12644

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development