CCL5 antibody
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- Target See all CCL5 Antibodies
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CCL5 antibody is un-conjugated
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Application
- Neutralization (Neut)
- Specificity
- Human RANTES
- Purification
- Protein A purified
- Immunogen
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E.coli derived recombinant Human RANTES.
Type of Immunogen: Cell extract - Isotype
- IgG2b
- Top Product
- Discover our top product CCL5 Primary Antibody
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- Application Notes
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Approved: Neut (5 - 7 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/mL), a concentration of 3-5 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with biotinylated antigen affinity purified anti-human RANTES (LS-C104720) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
- Alternative Name
- CCL5 / RANTES (CCL5 Products)
- Synonyms
- Rantes antibody, Scya5 antibody, D17S136E antibody, RANTES antibody, SCYA5 antibody, SIS-delta antibody, SISd antibody, TCP228 antibody, eoCP antibody, MuRantes antibody, C-C motif chemokine 5-like antibody, C-C motif chemokine ligand 5 antibody, chemokine (C-C motif) ligand 5 antibody, LOC100230935 antibody, CCL5 antibody, Ccl5 antibody
- Background
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Name/Gene ID: CCL5
Family: Intercrine
Synonyms: CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228 - Gene ID
- 6352
- UniProt
- P13501
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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