Nerve Growth Factor antibody

Catalog No. ABIN465164

The Mouse Monoclonal anti-Nerve Growth Factor antibody has been validated for WB, ELISA and Neut. It is suitable to detect Nerve Growth Factor in samples from Human.

Quick Overview for Nerve Growth Factor antibody (ABIN465164)

Target: Nerve Growth Factor (NGF)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Nerve Growth Factor Antibody

Specificity: Human beta-NGF

Purification: Protein G purified

Immunogen: E.coli derived recombinant Human beta-NGF.

Type of Immunogen: Cell extract

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (2 - 4 μg/mL), Neut (2.7 - 4 μg/mL), WB (4 - 8 μg/mL)

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (10 ng/mL), a concentration of 2.7-4 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 200 pg/mL of recombinant Human beta-NGF when used with biotinylated antigen affinity purified anti-Human beta-NGF (LS-C104735) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 4-8 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 4-8 ng/lane, under reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Background: Name/Gene ID: NGF
Family: NGF Beta

Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB

Gene ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size