CCL4 antibody (Biotin)

Catalog No. ABIN465194

This Rabbit Polyclonal antibody specifically detects CCL4 in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for CCL4 antibody (Biotin) (ABIN465194)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL4 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL4 Antibody (Biotin)

Specificity: Mouse MIP-1beta

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine MIP-1beta.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Murine MIP-1beta by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-1beta. To detect Murine MIP-1beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine MIP-1beta (LS-C104570) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-1beta. Western Blot: To detect Murine MIP-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine MIP-1beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: CCL4 / SYCA4

Background: Name/Gene ID: CCL4
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL4, ACT2, Act-2, AT744.1, C-C motif chemokine 4, Chemokine (C-C motif) ligand 4, CC chemokine ligand 4, HC21, LAG1, MIP1B, MIP1B1, LAG-1, SCYA2, Secreted protein G-26, SIS-gamma, MIP-1-beta, MIP-1-beta(1-69), Small-inducible cytokine A4, T-cell activation protein 2, G-26, Lymphocyte-activation gene 1, PAT 744, Protein H400, SCYA4

Gene ID: 6351

UniProt: P13236