CCL19 antibody (Biotin)

Catalog No. ABIN465252

The Rabbit Polyclonal anti-CCL19 antibody has been validated for WB, ELISA and Neut. It is suitable to detect CCL19 in samples from Human.

Quick Overview for CCL19 antibody (Biotin) (ABIN465252)

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL19 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL19 Antibody (Biotin)

Specificity: Human MIP-3beta

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIP-3beta.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human MIP-3beta by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-3beta. To detect Human MIP-3beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-3beta (LS-C104606) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-3beta. Western Blot: To detect Human MIP-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL19

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Alternative Name: CCL19 / MIP3-Beta

Background: Name/Gene ID: CCL19
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL19, C-C motif chemokine 19, CKb11, CC chemokine ligand 19, EBI1 ligand chemokine, EBI1-ligand chemokine, Exodus-3, ELC, MIP-3-beta, MIP3B, SCYA19, Small-inducible cytokine A19, Beta chemokine exodus-3, Beta-chemokine exodus-3, CK beta-11, MIP-3b

Gene ID: 6363

UniProt: Q99731

Pathways: Positive Regulation of Immune Effector Process