CCL25 antibody (Biotin)

Catalog No. ABIN465266

This Rabbit Polyclonal antibody specifically detects CCL25 in ELISA, WB and Neut. It exhibits reactivity toward Human.

Quick Overview for CCL25 antibody (Biotin) (ABIN465266)

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL25 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Product Details anti-CCL25 Antibody (Biotin)

Specificity: Human TECK

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TECK.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hTECK by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTECK. To detect hTECK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TECK (LS-C104396) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTECK. Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL25

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Alternative Name: TECK / CCL25

Background: Name/Gene ID: CCL25
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL25, C-C motif chemokine 25, Ck beta-15, TECK, Thymus-expressed chemokine, Small-inducible cytokine A25, TECKvar, Chemokine TECK, Ckb15, SCYA25, Thymus expressed chemokine

Gene ID: 6370

UniProt: O15444