IL-10 antibody

Catalog No. ABIN465277

This Rat Monoclonal antibody specifically detects IL-10 in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for IL-10 antibody (ABIN465277)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: 15B9-1-2

Product Details anti-IL-10 Antibody

Specificity: Mouse IL-10

Purification: Protein G purified

Immunogen: E.coli derived recombinant Murine IL-10.

Type of Immunogen: Cell extract

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (4 - 8 μg/mL), Neut (3 - 5 μg/mL), WB (1 - 2 μg/mL)

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-10 (0.5 ng/mL), a concentration of 3-5 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with biotinylated antigen affinity purified anti-murine IL-10 (LS-C104822) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1-2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2-4 ng/lane, under reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10

Background: Name/Gene ID: IL10
Family: Hormone

Synonyms: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF

Gene ID: 3586

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints