alpha Defensin 1 antibody (Biotin)

Catalog No. ABIN465279

The Goat Polyclonal anti-alpha Defensin 1 antibody has been validated for WB and ELISA. It is suitable to detect alpha Defensin 1 in samples from Human.

Quick Overview for alpha Defensin 1 antibody (Biotin) (ABIN465279)

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha Defensin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-alpha Defensin 1 Antibody (Biotin)

Specificity: Human NP-1

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human NP-1.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hNP-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hNP-1. To detect hNP-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human NP-1 (LS-B7416) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNP-1. Western Blot: To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for alpha Defensin 1

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Alternative Name: DEFA1 / HP1

Background: Name/Gene ID: DEFA1

Synonyms: DEFA1, DEFA2, Defensin, alpha 2, HP-1, HP1, Myeloid-related sequence, MRS, DEF1, Defensin, alpha 1, HNP-1, Neutrophil defensin 1

Gene ID: 1667

UniProt: P59665

Pathways: Cellular Response to Molecule of Bacterial Origin