CCL21 antibody (Biotin)

Catalog No. ABIN465506

This Rabbit Polyclonal antibody specifically detects CCL21 in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for CCL21 antibody (Biotin) (ABIN465506)

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL21 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL21 Antibody (Biotin)

Specificity: Mouse Exodus-2

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine Exodus-2.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mExodus-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mExodus-2. To detect mExodus-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine Exodus-2 (LS-C104634) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mExodus-2. Western Blot: To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL21

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Alternative Name: CCL21 / Exodus 2 / SLC

Background: Name/Gene ID: CCL21
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL21, 6Ckine, Beta chemokine exodus-2, C-C motif chemokine 21, Beta-chemokine exodus-2, CKb9, ECL, Small-inducible cytokine A21, SCYA21, SLC, Exodus-2, TCA4

Gene ID: 6366

UniProt: O00585

Pathways: Regulation of Actin Filament Polymerization