XCL1 antibody (Biotin)

Catalog No. ABIN465512

This Rabbit Polyclonal antibody specifically detects XCL1 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for XCL1 antibody (Biotin) (ABIN465512)

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XCL1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-XCL1 Antibody (Biotin)

Specificity: Human Lymphotactin

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Lymphotactin.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hLymphotactin by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hLymphotactin. To detect hLymphotactin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Lymphotactin (LS-C104661) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hLymphotactin. Western Blot: To detect hLymphotactin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLymphotactin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for XCL1

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Alternative Name: XCL1 / Lymphotactin

Background: Name/Gene ID: XCL1
Subfamily: Intercrine gamma
Family: Intercrine

Synonyms: XCL1, ATAC, C motif chemokine 1, Chemokine (C motif) ligand 1, Cytokine SCM-1, Cytokine scm, LPTN, SCM-1, SCM-1-alpha, SCM-1a, SCM1, SCYC1, Scyc2, Single cysteine motif 1a, Lymphotaxin, Xc chemokine ligand, LTN, Lymphotactin, SCM1A, Small-inducible cytokine C1, XC chemokine ligand 1

Gene ID: 6375

UniProt: P47992

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation