PDGF-BB Homodimer antibody (Biotin)

Catalog No. ABIN465516

The Rabbit Polyclonal anti-PDGF-BB Homodimer antibody is suitable to detect PDGF-BB Homodimer in samples from Human. It has been validated for WB and ELISA.

Quick Overview for PDGF-BB Homodimer antibody (Biotin) (ABIN465516)

Target: PDGF-BB Homodimer

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), ELISA

Product Details

Specificity: Human PDGF-BB

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human PDGF-BB.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hPDGF-BB by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PDGF-BB (LS-C104675) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details

Target: PDGF-BB Homodimer

Alternative Name: PDGF-BB

Background: Name/Gene ID: PDGFB
Family: PDGF

Synonyms: PDGFB, Becaplermin, PDGF-BB, PDGF-b, SSV, PDGF subunit B, PDGF-2, PDGF2, Proto-oncogene c-Sis, SIS, C-sis, PDGF, B chain

Gene ID: 5155

UniProt: P01127