CXCL5 antibody

Catalog No. ABIN465535

This Rabbit Polyclonal antibody specifically detects CXCL5 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for CXCL5 antibody (ABIN465535)

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL5 Antibody

Specificity: Human ENA-78

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human ENA-78.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (15 - 20 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1 μg/mL-2 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hENA-78 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human ENA-78 (LS-C104358) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hENA-78. Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CXCL5

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Alternative Name: CXCL5

Background: Name/Gene ID: CXCL5
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL5, C-X-C motif chemokine 5, ENA-78(1-78), GCP-2, ENA78, SCYB5, ENA-78, Small inducible cytokine B5, Small-inducible cytokine B5, LIX, AMCF-II

Gene ID: 6374

UniProt: P42830

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity