G-CSF antibody

Catalog No. ABIN465577

This Rabbit Polyclonal antibody specifically detects G-CSF in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for G-CSF antibody (ABIN465577)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G-CSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-G-CSF Antibody

Specificity: Human G-CSF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human G-CSF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (4 - 6 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hG-CSF (0.5 ng/mL), a concentration of 0.04-0.06 μg/mL of this antibody is required. ELISA: To detect hG-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (LS-C104674) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF. Western Blot: To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: CSF3 / G-CSF / GCSF

Background: Name/Gene ID: CSF3

Synonyms: CSF3, C17orf33, Filgrastim, G-CSF, GCSF, Pluripoietin, CSF3OS, Lenograstim

Gene ID: 1440

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization