Nerve Growth Factor antibody (Biotin)

Catalog No. ABIN465708

The Rabbit Polyclonal anti-Nerve Growth Factor antibody has been validated for WB, ELISA and Neut. It is suitable to detect Nerve Growth Factor in samples from Human.

Quick Overview for Nerve Growth Factor antibody (Biotin) (ABIN465708)

Target: Nerve Growth Factor (NGF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nerve Growth Factor antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Nerve Growth Factor Antibody (Biotin)

Specificity: Human beta-NGF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human beta-NGF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human beta-NGF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF. To detect Human beta-NGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human beta-NGF (LS-C104703) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Background: Name/Gene ID: NGF
Family: NGF Beta

Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB

Gene ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size