DEFB104A antibody (Biotin)

Catalog No. ABIN465716

This Rabbit Polyclonal antibody specifically detects DEFB104A in ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for DEFB104A antibody (Biotin) (ABIN465716)

Target: DEFB104A (Defensin, beta 104A (DEFB104A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEFB104A antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Product Details anti-DEFB104A Antibody (Biotin)

Specificity: Human BD-4

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human BD-4.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hBD-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-4. To detect hBD-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human BD-4 (LS-C104542) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-4. Western Blot: To detect hBD-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for DEFB104A

Target: DEFB104A (Defensin, beta 104A (DEFB104A))

Alternative Name: DEFB104A / DEFB4

Background: Name/Gene ID: DEFB104A

Synonyms: DEFB104A, Beta-defensin 4, BD-4, Beta-defensin 104, Defensin, beta 104, Defensin, beta 104A, Defensin, beta 4, DEFB4, HBD-4, DEFB-4, DEFB104

Gene ID: 140596

UniProt: Q8WTQ1