CXCL2 antibody

Catalog No. ABIN465914

The Rabbit Polyclonal anti-CXCL2 antibody is suitable to detect CXCL2 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for CXCL2 antibody (ABIN465914)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CXCL2 Antibody

Specificity: Human GRObeta

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human GRObeta.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human colon/rectum adenocarcinoma tissue. The recommended concentration is 1 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect Human GRO-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GRO-beta (LS-C104838) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human GRO-beta. Western Blot: To detect human GRO-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: MIP2 / GRO2 / CXCL2

Background: Name/Gene ID: CXCL2
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL2, C-X-C motif chemokine 2, CINC-2a, GROb, Growth-regulated protein beta, MIP-2a, MIP2-alpha, MGSA-b, MIP-2, SCYB2, MGSA beta, MIP2, Gro-beta, GRO2, GRO2 oncogene, MIP2A

Gene ID: 2920

UniProt: P19875

Pathways: Cellular Response to Molecule of Bacterial Origin