Caspase 3 antibody (N-Term) (Alexa Fluor 488)

Catalog No. ABIN470500

This Rabbit Polyclonal antibody specifically detects Caspase 3 in ELISA, ICC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Caspase 3 antibody (N-Term) (Alexa Fluor 488) (ABIN470500)

Target: Caspase 3 (CASP3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is conjugated to Alexa Fluor 488

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Caspase 3 Antibody (Alexa Fluor 488)

Binding Specificity: N-Term

Specificity: Detects endogenous levels of the large fragment (17/19 kD) of activated caspase-3 resulting from cleavage adjacent to aspartic acid 175. Does not recognize full length caspase-3 or other cleaved caspases. Species cross-reactivity: Mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3 (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:10), ICC (1:10), IF

Usage: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, and Immunofluorescence. Flow Cytometry: 1:10. Immunocytochemistry: 1:10. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, <0.1 % sodium azide, 2.0 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3 / Caspase 3

Background: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation