Desmoglein 1/2 antibody (C-Term)

Catalog No. ABIN470806

This Mouse Monoclonal antibody specifically detects Desmoglein 1/2 in IF, IHC, WB, IHC (fro) and IHC (p). It exhibits reactivity toward Cow, Human, Mouse and Rat.

Quick Overview for Desmoglein 1/2 antibody (C-Term) (ABIN470806)

Target: Desmoglein 1/2 (DSG1/2)

Reactivity: Cow, Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 1/2 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Desmoglein 1/2 Antibody

Binding Specificity: C-Term

Specificity: Species (tested so far) Human, bovine, rat, mouse. Recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells, does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165000 Desmoglein, 'band 3' polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ('RUD') of the cytoplasmic C-terminal part of the polypeptide. Reactivities on Cultured Cell Lines (tested so far): A-431, MCF-7, TR-146, PLC and various other human epithelial cell lines, bovine BMGE+H, BMGE-H, MDBK.

Purification: Protein A purified

Immunogen: Band 3 polypeptide of isolated desmosomes from bovine muzzle epidermis.

Type of Immunogen: Protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-Fr, IHC-P, WB

Usage: Suitable for use in Immunohistochemistry (frozen sections, paraffin-embedded tissue after microwave treatment), Western Blot, Cryo, Paraffin (Microwave), Immunofluorescence microscopy. Immunohistochemistry: 1:10 with PBS,  pH 7.4, Incubation Time 1 h at RT, extended with paraffin.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Desmoglein 1/2

Target: Desmoglein 1/2 (DSG1/2)

Alternative Name: Desmoglein 1/2 (DSG1/DSG2)

Background: This antibody detects: DSG1+2 / Desmoglein 1+2