FZD3 antibody (Cysteine-Rich Domain, Extracellular)

Catalog No. ABIN470847

This anti-FZD3 antibody is a Rat Monoclonal antibody detecting FZD3 in WB, ELISA, IHC, FACS and IHC (fro). Suitable for Human and Mouse.

Quick Overview for FZD3 antibody (Cysteine-Rich Domain, Extracellular) (ABIN470847)

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FZD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FZD3 Antibody

Binding Specificity: Cysteine-Rich Domain, Extracellular

Specificity: Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 or -8. Species cross-reactivity: Human.

Purification: Protein G purified

Immunogen: NS0-derived rmFrizzled-3 extracellular CRD domain.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-Fr, WB

Usage: Suitable for use in Direct ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. By Flow Cytometry and Immunohistochemistry, this antibody detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Flow Cytometry: Dilute this antibody to 5-10 μg/mL and add 10 mL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: A biotin conjugate of this antibody at a concentration of 8 μg/mL was used to detect Frizzled-3 in frozen mouse embryo sections. Western Blot: Use at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-3. Using a colorimetric detection system, the detection limit for rmFrizzled-3 is ~1 ng/lane under non-reducing and reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid frequent freeze thaws. Store undiluted.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for FZD3

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Alternative Name: FZD3 / Frizzled 3

Background: Name/Gene ID: FZD3
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3

Gene ID: 7976

UniProt: Q9NPG1

Pathways: WNT Signaling, Tube Formation