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FZD3 antibody (N-Term)

FZD3 Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Chicken, Hamster, Horse, Monkey, Rabbit, Bat IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048620
  • Target See all FZD3 Antibodies
    FZD3 (Frizzled Family Receptor 3 (FZD3))
    Binding Specificity
    • 15
    • 8
    • 8
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 58
    • 38
    • 23
    • 15
    • 5
    • 5
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Dog, Pig, Chicken, Hamster, Horse, Monkey, Rabbit, Bat
    Host
    • 51
    • 4
    • 3
    • 3
    Rabbit
    Clonality
    • 55
    • 6
    Polyclonal
    Conjugate
    • 30
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FZD3 antibody is un-conjugated
    Application
    • 57
    • 31
    • 10
    • 10
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human FZD3 / Frizzled 3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD6 (39 % ).
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%) Xenopus (89%) Platypus, Zebrafish (83%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 18 amino acid peptide from N-terminal extracellular domain of human FZD3 / Frizzled 3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%), Xenopus (89%), Platypus, Zebrafish (83%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product FZD3 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (3 - 5 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3-5 μg/mL.
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    FZD3 (Frizzled Family Receptor 3 (FZD3))
    Alternative Name
    FZD3 / Frizzled 3 (FZD3 Products)
    Synonyms
    Fz-3 antibody, FZ-3 antibody, fz3 antibody, Xfz3 antibody, frz3 antibody, hfz3 antibody, frz-3 antibody, frizzled3 antibody, frizzled-3 antibody, FZD3 antibody, AU020229 antibody, D930050A07Rik antibody, Fz3 antibody, fz9 antibody, fzd3 antibody, zg09 antibody, fzd3l antibody, frizzled class receptor 3 antibody, frizzled class receptor 3 L homeolog antibody, frizzled class receptor 3a antibody, frizzled class receptor 3b antibody, FZD3 antibody, fzd3 antibody, fzd3.L antibody, Fzd3 antibody, fzd3a antibody, fzd3b antibody
    Background
    Name/Gene ID: FZD3
    Subfamily: Frizzled
    Family: GPCR

    Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3
    Gene ID
    7976
    Pathways
    WNT Signaling, Tube Formation
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