FZD3 antibody (Cysteine-Rich Domain, Extracellular) (Biotin)

Catalog No. ABIN470848

This Rat Monoclonal antibody specifically detects FZD3 in WB, IHC, FACS, ICC and IHC (fro). It exhibits reactivity toward Human and Mouse.

Quick Overview for FZD3 antibody (Cysteine-Rich Domain, Extracellular) (Biotin) (ABIN470848)

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FZD3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FZD3 Antibody (Biotin)

Binding Specificity: Cysteine-Rich Domain, Extracellular

Specificity: Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 and -8. Species cross-reactivity: Human.

Purification: Protein G purified

Immunogen: NS0-derived rmFrizzled-3 extracellular CRD.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, ICC, IHC, IHC-Fr, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Detected Frizzled-3 by flow cytometry and immunohistochemistry on the differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Immunocytochemistry and flow cytometry experiments on human HEK 293 cells demonstrate that this antibody cross-reacts with human Frizzled-3. Western Blot: Use at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-3. The detection limit for rmFrizzled-3 is ~0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute this antibody to 0.2 mg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome. Immunocytochemistry: Use with the appropriate secondary reagents at 8 μg/mL to detect Frizzled-3 in cells or frozen mouse embryos. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1 mL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, BSA

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for FZD3

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Alternative Name: FZD3 / Frizzled 3

Background: Name/Gene ID: FZD3
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3

Gene ID: 7976

UniProt: Q9NPG1

Pathways: WNT Signaling, Tube Formation