GQ alpha/g11 alpha (C-Term) antibody

Catalog No. ABIN470875

This Rabbit Polyclonal antibody specifically detects in IP and WB. It exhibits reactivity toward Bat, Chicken, Cow, Dog, Goat, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Xenopus laevis and Zebrafish (Danio rerio).

Quick Overview for GQ alpha/g11 alpha (C-Term) antibody (ABIN470875)

Target: GQ alpha/g11 alpha

Reactivity: Bat, Chicken, Cow, Dog, Goat, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Binding Specificity: C-Term

Specificity: Specific for human Gq alpha and G11 alpha. Does not recognize other non-pertussis toxin-sensitive G proteins. Species cross-reactivity: Mouse and human.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Blood fluke, Sea anemone, Tick (100%) Water flea (90%).

Purification: Purified

Immunogen: KLH-conjugated, synthetic peptide (QLNLKEYNLV) corresponding to amino acids 350- 359 of G11 alpha or 344-353 of Gq alpha. This sequence is highly conserved among species. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Blood fluke, Sea anemone, Tick (100%), Water flea (90%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP (1:500 - 1:2000), WB

Usage: Suitable for use in Western Blot analysis and Immunoprecipitation. Immunoprecipitation: 1:500-1:2000 (detects Gq alpha and G11 alpha in 20 μg of mouse brain microsomes and membranes, detected Gq/11 a in 10 μg of mouse 3T3/NIH fibroblast soluble plasma membranes.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: GQ alpha/g11 alpha