M-Phase phosphoprotein Epitope (LTPLK Or FTPLQ) antibody

Catalog No. ABIN470947

This Mouse Monoclonal antibody specifically detects M-Phase phosphoprotein Epitope (LTPLK Or FTPLQ) in ICC, IP, ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for M-Phase phosphoprotein Epitope (LTPLK Or FTPLQ) antibody (ABIN470947)

Target: M-Phase phosphoprotein Epitope (LTPLK Or FTPLQ)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)

Product Details

Specificity: Recognizes a phosphorylated epitope (S/T) P found in phosphoproteins such as MAP2, HSP70, cdc25 and DNA topoisomerase IIa, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type. Species cross-reactivity: animals and plants.

Purification: Protein G purified

Immunogen: Mitotic human HeLa cell cytosolic lysate.

Type of Immunogen: Cell extract

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, ICC, IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunocytochemistry, ELISA and Immunoprecipitation. Immunoprecipitation: Reported by an independent laboratory. Positive control: Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7 % paraformaldehyde.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: M-Phase phosphoprotein Epitope (LTPLK Or FTPLQ)