Mps1 antibody (C-Term)

Catalog No. ABIN470948

The Mouse Monoclonal anti-Mps1 antibody has been validated for WB, IP and ICC. It is suitable to detect Mps1 in samples from Human.

Quick Overview for Mps1 antibody (C-Term) (ABIN470948)

Target: Mps1 (TTK)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Mps1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Mps1 Antibody

Binding Specificity: AA 3-856, C-Term

Specificity: Recognizes human Mps1, Mr ~97kD. This antibody is known to inhibit the autophosphorylation/ kinase activity of Mps1. Does not react with mouse.

Purification: Protein G purified

Immunogen: His-tagged fusion protein corresponding to aa3-856 of human Mps1, previously known at TTK/PYK.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate Mps1 from HeLa total cell extract. Immunocytochemistry: Has been reported by an independent laboratory to show positive immunostaining for Mps1 in U2OS or HeLa cells permeabilized with 0.5 % Triton-X-100 and fixed with methanol or paraformaldehyde.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Mps1

Target: Mps1 (TTK)

Alternative Name: MPS1 / TTK

Background: Name/Gene ID: TTK
Subfamily: TTK
Family: Protein Kinase

Synonyms: TTK, Cancer/testis antigen 96, ESK, Esk1, MPS1, MPS1L1, Protein kinase esk, PYT, TTK protein kinase, CT96, Monopolar spindle 1-like 1

Gene ID: 7272

UniProt: P33981