DYNC1I1 antibody (N-Term)

Catalog No. ABIN472619

The Mouse Monoclonal anti-DYNC1I1 antibody has been validated for WB, ICC, IF and IP. It is suitable to detect DYNC1I1 in samples from Rat, Mouse, Cow and Sheep.

Quick Overview for DYNC1I1 antibody (N-Term) (ABIN472619)

Target: DYNC1I1 (Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1))

Reactivity: Rat, Mouse, Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYNC1I1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-DYNC1I1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes all known forms of the Mr 74kD intermediate chain subunit (IC74) of mammalian cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes). Species cross-reactivity: Rat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid.

Purification: Purified

Immunogen: Bovine brain cytoplasmic dynein.

Type of Immunogen: Purified protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ICC (1:50 - 1:100), IF, IP, WB (1:1000 - 1:5000)

Usage: Suitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells. Western Blot: 1:1000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells. Immunoprecipitation: 10 μL/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for DYNC1I1

Target: DYNC1I1 (Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1))