Fibroblasts antibody

Catalog No. ABIN472810

This Mouse Monoclonal antibody specifically detects Fibroblasts in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for Fibroblasts antibody (ABIN472810)

Target: Fibroblasts

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibroblasts antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Fibroblasts Antibody

Specificity: Recognizes human fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue (e.g. fibrosarcomas) {Haynes et al, 1984}. Useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.

Purification: Ion exchange chromatography

Immunogen: Whole human thymic stoma cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:100), IF (1:100 - 1:200)

Usage: Suitable for use in Immunofluorescence, FACS, and the assessment of thymic epithelial culture contamination with fibroblasts and the identification of cells of mesodermal origin in the fetus. Immunofluorescence: 1:100-1:200 (acetone fixed tissue). FACS: 1:100 (fresh or acetone fixed tissue, do not use trypsin to prepare adherent cell lines, use a non-enzymatic detachment procedure.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 10 mM sodium azide, 1 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Fibroblasts

Target: Fibroblasts