FZD1 antibody (Extracellular Domain) (Biotin)

Catalog No. ABIN478743

This anti-FZD1 antibody is a Rat Monoclonal antibody detecting FZD1 in WB, ICC and FACS. Suitable for Human and Mouse.

Quick Overview for FZD1 antibody (Extracellular Domain) (Biotin) (ABIN478743)

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FZD1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-FZD1 Antibody (Biotin)

Binding Specificity: Extracellular Domain

Specificity: Recognizes mouse Frizzled-1 extracellular domain. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7 and -8. Detected Frizzled-1 on differentiated embryoid bodies derived from mouse embryonic cell line D3 by Flow Cytometry. Species cross-reactivity: Human.

Purification: Protein G purified

Immunogen: NS0-derived rmFrizzled-1 extracellular domain.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, ICC, WB

Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 10 μL of this solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 mL of a 25 mg/mL stock solution of a secondary developing reagent such as streptavidin conjugated to a fluorochrome. Immunocytochemistry: Used with appropriate secondary reagents at 5-10 μg/mL in 4 % . paraformaldehyde-fixed cells. Western Blot: Use at 1-2 μg/mL with appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5 ng/lane under non-reducing and reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, BSA.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for FZD1

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Alternative Name: FZD1 / Frizzled 1

Background: Name/Gene ID: FZD1
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1

Gene ID: 8321

UniProt: Q9UP38

Pathways: WNT Signaling, Asymmetric Protein Localization