FZD7 antibody (Extracellular Domain)

Catalog No. ABIN478765

This Rat Monoclonal antibody specifically detects FZD7 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for FZD7 antibody (Extracellular Domain) (ABIN478765)

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FZD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FZD7 Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes mouse Frizzled-7. No cross-reactivity was observed with rmFrizzled-1, -2, -3, -4, -6 or -8.

Purification: Protein G purified

Immunogen: NS0-derived rmFrizzled-7 extracellular domain.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Direct ELISA. Western Blot: Use at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-7. Using a colorimetric detection system, the detection limit for rmFrizzled-7 is ~1 ng/lane and ~5 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: Use at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-7. The detection limit for rmFrizzled-7 is ~5 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for FZD7

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Alternative Name: FZD7 / Frizzled 7

Background: Name/Gene ID: FZD7
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD7, Frizzled family receptor 7, Frizzled homolog 7, Frizzled 7, Frizzled-7, FzE3, Fz-7, HFz7

Gene ID: 8324

UniProt: O75084

Pathways: WNT Signaling, Stem Cell Maintenance