Keratin Basic antibody

Catalog No. ABIN478948

This Mouse Monoclonal antibody specifically detects in WB, IF, IHC, IHC (p), IHC (fro) and ELISA. It exhibits reactivity toward Human, Rat, Mouse and Rabbit.

Quick Overview for Keratin Basic antibody (ABIN478948)

Target: Keratin Basic

Reactivity: Human, Rat, Mouse, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Clone: AE3

Product Details

Specificity: Recognizes the 65-67, 64, 59, 58, 56 and 52kD keratins of the basic subfamily. Species Reactivity: Human, Monkey, bovine, Rabbit, Mouse, Rat, Chicken.

Purification: Purified

Immunogen: Human epidermal keratin.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-Fr, IHC-P (1 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in ELISA, Immunofluorescence, Western Blot, Immunohistochemistry. Western Blot: 1 μg/mL. Immunohistochemistry: 4 μg/mL. Frozen & formalin/paraffin. For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 10 min at 37?C or Protease XXV at 1 mg/mL PBS for 5 minutes at 37?C.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details

Target: Keratin Basic

Alternative Name: Basic Cytokeratin