MHC Class I H-2Db (Chain alpha 3), (Domain alpha 3) antibody

Catalog No. ABIN479215

Product Details

Target: MHC Class I H-2Db

Binding Specificity: Chain alpha 3, Domain alpha 3

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Cytotoxicity Test (CyTox), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes an epitope in the alpha3 domain of H-2Db. It cross-reacts with the alpha3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. Has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.

Purification: Purified

Immunogen: C3H. SW mouse splenocytes.

Type of Immunogen: Cells

Clone: 28-14-8

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: Ctox, Flo, IHC, IHC-Fr

Usage: Flow cytometry. Immunohistochemistry (frozen tissue sections). Complement-mediated cytotoxicity. Flow Cytometry: 1 μg/1 x10⁶.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM borate buffered saline,  pH 8.0. No preservatives or amine-containing buffer salts added.

Preservative: Without preservative

Target Details

Target: MHC Class I H-2Db